CCL22 ELISA Kit (Mouse)

Mean Inter-assay CV%:

7732-18-5

Chemokine (C-C motif) ligand 22

ABCD-; ABCD-1; activated B and dendritic cell-derived; CC chemokine ABCD-1; C-C motif chemokine 22; DCBCK; dendritic cell and B cell derived chemokine; MDC; Scya; Scya22; SMALL INDUCIBLE CYTOKINE A22 PRECURSOR (CC CHEMOKINE ABCD-1) (ACTIVATED B AND DENDRITIC CELL-DERIVED) ; small inducible cytokine subfamily A, member 22; small inducible cytokine subfamily A22; small-inducible cytokine A22.

20299

NP_033163.1

Mouse, Mus musculus

C-C motif chemokine 22

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology Ccl22 ELISA Kit (Mouse) (OKEH04046) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl22 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl22 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl22 captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

15.6-1000pg/mL

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

7.87 pg/mL

Store as indicated in product manual.

10 kDa

Natural and recombinant Mouse C-C motif chemokine 22

92

Ccl22

O88430

Ccl22