Products for Research Use Only
RegisterLogin

CCL22 ELISA Kit (Rat)

CAT: 0247-OKWB00363-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKWB00363-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Aliases
Mdc, MGC108943, Scya22
Reactivity
Rat|Rattus norvegicus
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology Ccl22 ELISA Kit (Rat) (OKWB00363) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl22 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl22 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl22 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
15.625-1000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 91-101 94 EDTA Plasma (n=5) 88-104 95 Heparin Plasma (n=5) 88-102 93 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 91-101 94 Serum (n=5) 91-101 94 EDTA Plasma (n=5) 88-104 95 EDTA Plasma (n=5) 88-104 95 Heparin Plasma (n=5) 88-102 93 Heparin Plasma (n=5) 88-102 93
Sensitivity
9.4 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Ccl22 Microplate 96 Wells (12 x 8 Well strips) Ccl22 Lyophilized Standard 2 x 1 ng 100X Ccl22 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Ccl22 Microplate 96 Wells (12 x 8 Well strips) Ccl22 Microplate 96 Wells (12 x 8 Well strips) Ccl22 Lyophilized Standard 2 x 1 ng Ccl22 Lyophilized Standard 2 x 1 ng 100X Ccl22 Biotinylated Detector Anitbody 1 x 120 uL 100X Ccl22 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
CCL22
Protein Name
C-C motif chemokine
Gene Name URL
CCL22

Related Products

CatalogName
OKEH04046-96WCCL22 ELISA Kit (Mouse)
OKBB01492-96WCCL22 ELISA Kit (Human)
OKAN04750CCL22 ELISA Kit (Mouse) (OKAN04750)
OKEH04046CCL22 ELISA Kit (Mouse) (OKEH04046)
OKBB01492CCL22 ELISA Kit (Human) (OKBB01492)
OKCD05909CCL22 ELISA Kit (Mouse) (OKCD05909)
OKEH04005CCL22 ELISA Kit (Human) (OKEH04005)
OKAN04723CCL22 ELISA Kit (Human) (OKAN04723)
OKCD05908CCL22 ELISA Kit (Human) (OKCD05908)
OKCD03745-96WCCL22 Chemi-Luminescent ELISA Kit (Human)

Alternative Products

CatalogName
GTR18806576-01Rat CCL22 ELISA Kit
EF017880Rat MDC/CCL22 ELISA Kit
GTR18827999-01Rat CCL22 ELISA Kit (Ready to Use)
GTR18856925-01Rat Macrophage-Derived Chemokine (MDC/CCL22) ELISA Kit
YP-W34373-01Rat Macrophage-Derived Chemokine 22 (CCL22) ELISA Kit
MBS2509174-01Rat MDC/CCL22 (Macrophage-Derived Chemokine) ELISA Kit
abx255808-01Rat Macrophage Derived Chemokine / MDC (CCL22) ELISA Kit
E8989Macrophage Derived Chemokine (MDC/CCL22) Rat ELISA Kit
E-EL-R0609ELISA kit for Rat MDC/CCL22 (Macrophage-Derived Chemokine)
SYP-R0171-01Rat MDC/CCL22(Macrophage-Derived Chemokine) ELISA Kit

Popular Products