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AGTR1B ELISA Kit (Mouse)

CAT: 0247-OKEH03728-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03728-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Angiotensin II receptor, type 1b
Gene Aliases
Agtr-; Agtr-1b; angiotensin II type-1B receptor; Angtr; Angtr-1b; AT1; AT1B; AT2R1B; AT3; type-1B angiotensin II receptor.
Gene ID
11608
Accession Number
NP_780295.2
Reactivity
Mouse, Mus musculus
Target
Type-1B angiotensin II receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology AGTR1B ELISA Kit (Mouse) (OKEH03728) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Agtr1b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Agtr1b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Agtr1b captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.086 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
41 kDa
Specificity
Natural and recombinant Mouse Type-1B angiotensin II receptor
Protein Length
359
NCBI Gene Symbol
Agtr1b
Protein Name
P29755
Gene Name URL
Agtr1b
Nucleotide Accession Number
NM_175086.3

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