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AGTR1B ELISA Kit (Rat)

CAT: 0247-OKEH05966-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05966-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Angiotensin II receptor, type 1b
Gene Aliases
1
Gene ID
81638
Accession Number
NP_112271.2
Reactivity
Rat
Target
Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Agtr1b ELISA Kit (Rat) (OKEH05966) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Agtr1b has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Agtr1b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Agtr1b captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156 - 10ng/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 101%
Sensitivity
0.087 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Type-1B angiotensin II receptor
Protein Length
359
NCBI Gene Symbol
AGTR1B
Protein Name
Type-1B angiotensin II receptor
Gene Name URL
AGTR1B
Nucleotide Accession Number
NM_031009.2

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