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AGTR2 ELISA Kit (Mouse)

CAT: 0247-OKEH05762-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05762-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Angiotensin II receptor, type 2
Gene Aliases
AI316812; angiotensin II type-2 receptor; AT2 receptor; AW107640; type-2 angiotensin II receptor.
Gene ID
11609
Accession Number
NP_031455.1
Reactivity
Mouse, Mus musculus
Target
Type-2 angiotensin II receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Agtr2 ELISA Kit (Mouse) (OKEH05762) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Agtr2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Agtr2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Agtr2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 107.3%
Sensitivity
8.06 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Type-2 angiotensin II receptor
Protein Length
363
NCBI Gene Symbol
Agtr2
Protein Name
P35374
Gene Name URL
Agtr2
Nucleotide Accession Number
NM_007429.5

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