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TAP ELISA Kit (Mouse)

CAT: 0247-OKEH03669-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03669-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)
Gene Aliases
T, RI, Y3, Ham, MTP, PSF, TAP, APT1, Abcb, Ham-, Ham1, MTP1, PSF1, ABC17, Abcb2, Ham-1, RING4, Tap-1
Gene ID
21354
Accession Number
NP_038711.2
Reactivity
Mouse
Target
Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TAP ELISA Kit (Mouse) (OKEH03669) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TAP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for TAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TAP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115%
Sensitivity
0.08 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
79 kDa
Specificity
Natural and recombinant Mouse Trypsinogen Activation Peptide, TAP
Protein Length
724
NCBI Gene Symbol
TAP
Protein Name
Antigen peptide transporter 1
Gene Name URL
TAP
Nucleotide Accession Number
NM_013683.2

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