TAP2 ELISA Kit (Human)

CAT: 0247-OKEH07514-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH07514-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Transporter 2, ATP binding cassette subfamily B member
Gene Aliases
ABC transporter, MHC 2; ABC18; ABCB3; antigen peptide transporter 2; APT2; ATP-binding cassette sub-family B member 3; ATP-binding cassette, sub-family B (MDR/TAP), member 3; D6S217E; peptide supply factor 2; peptide transporter involved in antigen processing 2; peptide transporter PSF2; Peptide transporter TAP2; PSF2; PSF-2; really interesting new gene 11 protein; RING11; transporter 2, ABC (ATP binding cassette) ; transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) .
Gene ID
6891
Accession Number
NP_000535.3
Reactivity
Homo sapiens, Human
Target
Antigen peptide transporter 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology TAP2 ELISA Kit (OKEH07514) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-TAP2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TAP2 and incubated. The TAP2 found in the sample or standards competes with the biotinylated TAP2 for limited binding sites on the immobilized anti-TAP2 antibody. Excess unbound biotinylated TAP2 and sample or standard TAP2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TAP2 captured in the well and inversely proportional to the amount of TAP2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113% (n = 20)
Sensitivity
0.307ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Antigen peptide transporter 2
NCBI Gene Symbol
TAP2
Protein Name
Q03519
Gene Name URL
TAP2
Nucleotide Accession Number
NM_000544.3

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