TAP2 ELISA Kit (Rat)

CAT: 0247-OKEH07516-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH07516-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Transporter 2, ATP binding cassette subfamily B member
Gene Aliases
Abcb3; antigen peptide transporter 2; APT2; ATP-binding cassette sub-family B member 3; Cim; Transporter 2 ABC (ATP binding cassette) ; transporter 2 ATP-binding cassette sub-family B; transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) .
Gene ID
24812
Accession Number
NP_114445.2
Reactivity
Rat, Rattus norvegicus
Target
Antigen peptide transporter 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TAP2 ELISA Kit (Rat) (OKEH07516) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TAP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TAP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TAP2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113% (n = 20)
Sensitivity
0.53ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Antigen peptide transporter 2
NCBI Gene Symbol
Tap2
Protein Name
P36372
Gene Name URL
Tap2
Nucleotide Accession Number
NM_032056.3

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