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BACE2 ELISA Kit (Rat)

CAT: 0247-OKEH03415-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03415-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Beta-secretase 2
Gene Aliases
Beta-secretase 2; beta-site amyloid precursor protein cleaving enzyme 2; beta-site APP-cleaving enzyme 2; memapsin-1; membrane-associated aspartic protease 1; theta-secretase.
Gene ID
288227
Accession Number
NP_001002802.1
Reactivity
Rat, Rattus norvegicus
Target
Beta-secretase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BACE2 ELISA Kit (Rat) (OKEH03415) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bace2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Bace2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bace2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Sensitivity
0.177 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
56 kDa
Specificity
Natural and recombinant Rat Beta-secretase 2
Protein Length
514
NCBI Gene Symbol
Bace2
Protein Name
Q6IE75
Gene Name URL
Bace2
Nucleotide Accession Number
NM_001002802.1

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