BACE2 ELISA Kit (Human)

CAT: 0247-OKEH01293-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH01293-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Beta-secretase 2
Gene Aliases
56 kDa aspartic-like protease; AEPLC; ALP56; ASP1; ASP21; Aspartic-like protease 56 kDa; aspartyl protease 1; BAE2; beta-secretase 2; beta-site amyloid beta A4 precursor protein-cleaving enzyme 2; Beta-site amyloid precursor protein cleaving enzyme 2; beta-site APP-cleaving enzyme 2; CDA13; CEAP1; Down region aspartic protease; Down syndrome region aspartic protease; DRAP; memapsin-1; membrane-associated aspartic protease 1; SLCO3A1/BACE2 fusion; theta-secretase; transmembrane aspartic proteinase Asp1.
Gene ID
25825
Accession Number
NP_036237.2
Reactivity
Homo sapiens, Human
Target
Beta-secretase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BACE2 ELISA Kit (Human) (OKEH01293) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BACE2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BACE2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BACE2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
56 kDa
Specificity
Natural and recombinant Human Beta-secretase 2
Protein Length
518
NCBI Gene Symbol
BACE2
Protein Name
Q9Y5Z0
Gene Name URL
BACE2
Nucleotide Accession Number
NM_012105.4

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