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CAMP ELISA Kit (Mouse)

CAT: 0247-OKEH03274-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03274-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Cathelicidin antimicrobial peptide
Gene Aliases
C; CAP; CAP18; cathelicidin antimicrobial peptide; cathelin-like protein; cathelin-related antimicrobial peptide; CLP; Cnlp; Cr; Cramp; FAL; FALL39; MC; MCLP.
Gene ID
12796
Accession Number
NP_034051.2
Reactivity
Mouse, Mus musculus
Target
Cathelicidin antimicrobial peptide
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology CAMP ELISA Kit (Mouse) (OKEH03274) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Camp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Camp and incubated. The Camp found in the sample or standards competes with the biotinylated Camp for limited binding sites on the immobilized anti-Mouse Camp antibody. Excess unbound biotinylated Camp and sample or standard Camp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Camp captured in the well and inversely proportional to the amount of Camp which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Recovery
Spike Average Recovery: 80%
Sensitivity
0.091 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
19 kDa
Specificity
Natural and recombinant Mouse Cathelin-related antimicrobial peptide
Protein Length
172
NCBI Gene Symbol
Camp
Protein Name
P51437
Gene Name URL
Camp
Nucleotide Accession Number
NM_009921.2

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