CAMP ELISA Kit

• Reproducibility:

  Sample Cyclic AMP Conc. (pmol/mL) %CV 1 11.2 3.9 2 3.6 8.3 Sample Cyclic AMP Conc. (pmol/mL) %CV Sample Cyclic AMP Conc. (pmol/mL) %CV 1 11.2 3.9 1 11.2 3.9 2 3.6 8.3 2 3.6 8.3 Sample Cyclic AMP Conc. (pmol/mL) %CV 1 6.5 8.1 2 2.1 13.0 Sample Cyclic AMP Conc. (pmol/mL) %CV Sample Cyclic AMP Conc. (pmol/mL) %CV 1 6.5 8.1 1 6.5 8.1 2 2.1 13.0 2 2.1 13.0 Sample Cyclic AMP Conc. (pmol/mL) %CV 1 1.32 8.4 2 0.39 10.9 Sample Cyclic AMP Conc. (pmol/mL) %CV Sample Cyclic AMP Conc. (pmol/mL) %CV 1 1.32 8.4 1 1.32 8.4 2 0.39 10.9 2 0.39 10.9 Sample Cyclic AMP Conc. (pmol/mL) %CV 1 1.02 10.1 2 0.32 16.5 Sample Cyclic AMP Conc. (pmol/mL) %CV Sample Cyclic AMP Conc. (pmol/mL) %CV 1 1.02 10.1 1 1.02 10.1 2 0.32 16.5 2 0.32 16.5

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked immunosorbent assay

• Detection Method:

  Chemiluminescent

• Assay Principle:

  The DetectX® Direct High Sensitivity Cyclic AMP (cAMP) Chemiluminescent Immunoassay Kit is designed to quantitatively measure cAMP present in lysed cells, EDTA and heparin plasma, urine, saliva and tissue culture media samples. Please read the complete kit insert before performing this assay. For tissue samples, saliva and urine, where the levels of cAMP are expected to be relatively high, the regular format for the assay can be used. For plasma samples and some dilute cell lysates an optional acetylation protocol should be used. This kit can measure as little as 1 femtomol cAMP per sample. The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent, which contains special additives and stabilizers, for cAMP measurement. This allows plasma, urine and saliva samples to be read in an identical manner to lysed cells. Acidified samples of cAMP are stable and endogenous phosphodiesterases are inactivated in the Sample Diluent. A cAMP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. A white microtiter plate coated with an antibody to capture sheep IgG is provided. Prior to the addition of any samples or standards a neutralizing Plate Primer solution is added to all the used wells. Standards or diluted samples, either with or without acetylation, are pipetted into the primed wells. A cAMP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to cAMP to each well. After a 2 hour incubation, the plate is washed and the chemiluminescent substrate is added. The substrate reacts with the bound cAMP-peroxidase conjugate to produce light The generated light is detected in a microtiter plate reader capable of reading luminescence. The concentration of the cAMP in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell Lysates, Saliva, Urine, EDTA and Heparin Plasma, Tissue Culture Media

• Sensitivity:

  Regular: Sensitivity was determined as 0.119 pmol/mL. This is equivalent to 5.95 fmol cAMP per well. Acetylated: Sensitivity was determined as 0.015 pmol/mL. This is equivalent to 0.75 fmol cAMP per sample.

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Coated White 96 Well Plates 1 or 5 Each Cyclic AMP Standard 125 uL DetectX® Cyclic AMP Antibody 3 mL or 13 mL DetectX® Cyclic AMP Conjugate Concentrate 60 uL or 260 uL Conjugate Diluent 3 mL or 13 mL Sample Diluent Concentrate 12 mL or 60 mL Plate Primer 25 mL Acetic Anhydride 2 mL Triethylamine 4 mL Wash Buffer Concentrate 30 mL or 125 mL Substrate Solution A 6mL or 28 mL Substrate Solution B 6mL or 28 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated White 96 Well Plates 1 or 5 Each Coated White 96 Well Plates 1 or 5 Each Cyclic AMP Standard 125 uL Cyclic AMP Standard 125 uL DetectX® Cyclic AMP Antibody 3 mL or 13 mL DetectX® Cyclic AMP Antibody 3 mL or 13 mL DetectX® Cyclic AMP Conjugate Concentrate 60 uL or 260 uL DetectX® Cyclic AMP Conjugate Concentrate 60 uL or 260 uL Conjugate Diluent 3 mL or 13 mL Conjugate Diluent 3 mL or 13 mL Sample Diluent Concentrate 12 mL or 60 mL Sample Diluent Concentrate 12 mL or 60 mL Plate Primer 25 mL Plate Primer 25 mL Acetic Anhydride 2 mL Acetic Anhydride 2 mL Triethylamine 4 mL Triethylamine 4 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL Substrate Solution A 6mL or 28 mL Substrate Solution A 6mL or 28 mL Substrate Solution B 6mL or 28 mL Substrate Solution B 6mL or 28 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each

• NCBI Gene Symbol:

  CAMP

• Gene Name URL:

  CAMP

• CAS Number:

  7732-18-5