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CAMP ELISA Kit (Human)

CAT: 0247-OKCA00619-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00619-96WSize:96 Well
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Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Cathelicidin antimicrobial peptide
Gene Aliases
LL37, CAP18, CRAMP, HSD26, CAP-18, FALL39, FALL-39
Gene ID
820
Accession Number
NP_004336.3
Reactivity
Human
Target
Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Antibacterial Peptide LL-37 ELISA Kit (Human) (OKCA00619) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Antibacterial Peptide LL-37 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Antibacterial Peptide LL-37 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Antibacterial Peptide LL-37 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Recovery
_x005F Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 94 89-98 _x005F EDTA Plasma (n=4) 104 92-112 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 94 89-98 Serum (n=5) 94 89-98 EDTA Plasma (n=4) 104 92-112 EDTA Plasma (n=4) 104 92-112
Sensitivity
0.039 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CAMP
Protein Name
Cathelicidin antimicrobial peptide
Gene Name URL
CAMP
Nucleotide Accession Number
NM_004345.4

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