Products for Research Use Only
RegisterLogin

DEFB2 ELISA Kit (Mouse)

CAT: 0247-OKEH03104-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH03104-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Defensin beta 2
Gene Aliases
BD-2; beta-defensin 2; Defensin, beta 2.
Gene ID
13215
Accession Number
NP_034160.1
Reactivity
Mouse, Mus musculus
Target
Beta-defensin 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DEFB2 ELISA Kit (Mouse) (OKEH03104) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse Defb2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Defb2 and incubated. The Defb2 found in the sample or standards competes with the biotinylated Defb2 for limited binding sites on the immobilized anti-mouse Defb2 antibody. Excess unbound biotinylated Defb2 and sample or standard Defb2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Defb2 captured in the well and inversely proportional to the amount of Defb2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
62.5-4000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 94%
Sensitivity
31.3 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
8 kDa
Specificity
Natural and recombinant Mouse Beta-defensin 2
Protein Length
71
NCBI Gene Symbol
Defb2
Protein Name
P82020
Gene Name URL
Defb2
Nucleotide Accession Number
NM_010030.1

Related Products

CatalogName
OKEH03834-96WDEFB2 ELISA Kit (Bovine)
OKEH03834DEFB2 ELISA Kit (Bovine) (OKEH03834)
OACD03028-1MLDEFB2 Antibody
OACD03028-100ULDEFB2 Antibody
OPCD02716-50UGDEFB2 Recombinant Protein
OPCD02716-200UGDEFB2 Recombinant Protein
OPCD02716-10UGDEFB2 Recombinant Protein
OKEH07209-96WDEFB3 ELISA Kit (Mouse)
OKEH04306-96WDEFB1 ELISA Kit (Mouse)
OKEH03085-96WDEFB4 ELISA Kit (Mouse)

Alternative Products

CatalogName
E045698Mouse DEFb2 ELISA Kit
GTR18831631-01Mouse DEFb2 ELISA Kit (Ready to Use)
E1738Mo-01Mouse Beta-defensin 2, DEFB2 ELISA KIT
MBS458833-01Mouse Defensin Beta 2 (DEFb2) ELISA Kit
C8414Beta-Defensin 2 (DEFb2) Mouse ELISA Kit
KLM1738GENLISA™ Mouse Defensin Beta 2 (DEFb2) ELISA
E0391MoMouse Defb2/Beta-defensin 2 ELISA Kit
abx153908-01Mouse Beta-Defensin 2 (DEFB2) ELISA Kit
MBS8801864-01Mouse DEFb2 (Defensin Beta 2) ELISA Kit
BG-MUS10778Mouse Defensin Beta 2 Defb2 ELISA Kit

Popular Products