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DEFB4 ELISA Kit (Mouse)

CAT: 0247-OKEH03085-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH03085-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Defensin beta 4
Gene Aliases
2310001F05Rik; BD-4; beta-defensin 4; Defensin, beta 4.
Gene ID
56519
Accession Number
NP_062702.1
Reactivity
Mouse, Mus musculus
Target
Beta-defensin 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DEFB4 ELISA Kit (Mouse) (OKEH03085) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Defb4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Defb4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Defb4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.415 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
7 kDa
Specificity
Natural and recombinant Mouse Beta-defensin 4
Protein Length
63
NCBI Gene Symbol
Defb4
Protein Name
P82019
Gene Name URL
Defb4
Nucleotide Accession Number
NM_019728.4

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