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H2AA ELISA Kit (Mouse)

CAT: 0247-OKEH02479-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH02479-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Histocompatibility 2, class II antigen A, alpha
Gene Aliases
A; Aalpha; H-2; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-U alpha chain; H2Aa; H-2Aa; histocompatibility 2, class II antigen A, alpha; histocompatibility 2, class II antigen A, alpha, promoter 1; I; I-; Ia; Ia1; Ia-1; IAalpha; I-Aalpha; major histocompatibility protein class II alpha chain; MHC class II antigen alpha chain; MHC-II I-A (d) alpha.
Gene ID
14960
Accession Number
NP_034508.2
Reactivity
Mouse, Mus musculus
Target
H-2 class II histocompatibility antigen, A-B alpha chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology H2AA ELISA Kit (Mouse) (OKEH02479) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse H2AA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated H2AA and incubated. The H2AA found in the sample or standards competes with the biotinylated H2AA for limited binding sites on the immobilized anti-Mouse H2AA antibody. Excess unbound biotinylated H2AA and sample or standard H2AA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated H2AA captured in the well and inversely proportional to the amount of H2AA which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
0.325 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 kDa
Specificity
Natural and recombinant Mouse H-2 class II histocompatibility antigen, A-B alpha chain
Protein Length
256
NCBI Gene Symbol
H2-Aa
Protein Name
P14434
Gene Name URL
H2-Aa
Nucleotide Accession Number
NM_010378.2

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