PLIN4 ELISA Kit (Mouse)

CAT: 0247-OKCD08592-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD08592-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Perilipin 4 (PLIN4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Perilipin 4 (PLIN4) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Perilipin 4
Gene Aliases
Adipocyte protein S3-12; mKIAA1881; perilipin-4; plasma membrane associated protein, S3-12; S3-1; S3-12.
Gene ID
57435
Accession Number
NP_065593.2
Reactivity
Mouse|Mus musculus
Target
May play a role in triacylglycerol packaging into adipocytes. May function as a coat protein involved in the biogenesis of lipid droplets.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Perilipin 4 (PLIN4) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Perilipin 4 (PLIN4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Perilipin 4 (PLIN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Perilipin 4 (PLIN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.064ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount PLIN4 Microplate 96 Wells (12 x 8 Well strips) PLIN4 Lyophilized Standard 2 100X PLIN4 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount PLIN4 Microplate 96 Wells (12 x 8 Well strips) PLIN4 Microplate 96 Wells (12 x 8 Well strips) PLIN4 Lyophilized Standard 2 PLIN4 Lyophilized Standard 2 100X PLIN4 HRP-Detector Anitbody 1 x 120 uL 100X PLIN4 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL
NCBI Gene Symbol
Plin4
Protein Name
Perilipin-4
Gene Name URL
Plin4
Nucleotide Accession Number
NM_020568.3

Related Products

CatalogName
OKCD00929-96WPLIN4 ELISA Kit (Human)
OKCD00929PLIN4 ELISA Kit (Human) (OKCD00929)
OACD00799-1MLPLIN4 Antibody
OACD00799-100ULPLIN4 Antibody
OPCD00487-200UGPLIN4 Recombinant Protein
OPCA03550-100UGPLIN4 Recombinant Protein
OPCD00487-50UGPLIN4 Recombinant Protein
OPCA03550-20UGPLIN4 Recombinant Protein
OPCA03692-100UGPLIN4 Recombinant Protein
OPCA03692-20UGPLIN4 Recombinant Protein

Alternative Products

CatalogName
E063230Mouse PLIN4 ELISA Kit
EM23006Mouse PLIN4 (Perilipin 4) ELISA Kit
MBS2705350-01Mouse Perilipin 4 (PLIN4) ELISA Kit
KLM6139GENLISA™ Mouse Perilipin 4 (PLIN4) ELISA
ELI-45335mMouse Perilipin-4, Plin4 ELISA KIT
F7685Perilipin 4 (PLIN4) Mouse ELISA Kit
027351Perilipin 4 (PLIN4) BioAssayTM ELISA Kit (Mouse)
CRM6024-01PLIN4 siRNA (Mouse)
37014054PLIN4 Adenovirus (Mouse)
abx928956-01Mouse PLIN4 siRNA

Popular Products