PDHA2 ELISA Kit (Rat)

7732-18-5

Pyruvate dehydrogenase E1 subunit alpha 2

Pdhal; PDHE1-A type II; pyruvate dehydrogenase (lipoamide) alpha 2; pyruvate dehydrogenase alpha 2; pyruvate dehydrogenase E1 alpha 2 subunit; pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial.

117098

NP_446446.1

Rat, Rattus norvegicus

Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology PDHA2 ELISA Kit (Rat) (OKEH02071) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDHA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDHA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDHA2 captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

78-5000pg/mL

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

39 pg/mL

Store as indicated in product manual.

43 kDa

Natural and recombinant Rat Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial

391

Pdha2

Q06437

Pdha2