PDHA2 ELISA Kit (Human)

CAT: 0247-OKEH02070-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH02070-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Pyruvate dehydrogenase E1 subunit alpha 2
Gene Aliases
PDHAL; PDHE1-A type II; pyruvate dehydrogenase (lipoamide) alpha 2; pyruvate dehydrogenase alpha 2; pyruvate dehydrogenase E1 alpha 2 subunit; pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; pyruvate dehydrogenase, E1-alpha polypeptide, testis specific.
Gene ID
5161
Accession Number
NP_005381.1
Reactivity
Homo sapiens, Human
Target
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PDHA2 ELISA Kit (Human) (OKEH02070) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDHA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDHA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDHA2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.067 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
43 kDa
Specificity
Natural and recombinant Human Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
Protein Length
388
NCBI Gene Symbol
PDHA2
Protein Name
P29803
Gene Name URL
PDHA2
Nucleotide Accession Number
NM_005390.4

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