PDHA2 ELISA Kit (Mouse)

CAT: 0247-OKEH05738-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH05738-96WSize:96 Tests
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Pyruvate dehydrogenase E1 alpha 2
Gene Aliases
Pd; Pdhal; PDHE1-A type II; PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, TESTIS-SPECIFIC FORM, MITOCHONDRIAL PRECURSOR (PDHE1-A TYPE II) ; pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial.
Gene ID
18598
Accession Number
NP_032837.1
Reactivity
Mouse, Mus musculus
Target
Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pdha2 ELISA Kit (Mouse) (OKEH05738) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pdha2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pdha2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pdha2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 91%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
Protein Length
391
NCBI Gene Symbol
Pdha2
Protein Name
P35487
Gene Name URL
Pdha2
Nucleotide Accession Number
NM_008811.2

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