DDT ELISA Kit (Human)

7732-18-5

D-dopachrome tautomerase

DDCT; D-dopachrome decarboxylase; D-dopachrome tautomerase; D-DT; MIF2; MIF-2; phenylpyruvate tautomerase II.

1652

NP_001077861.1

Homo sapiens, Human

D-dopachrome decarboxylase

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Aviva Systems Biology DDT ELISA Kit (Human) (OKEH00729) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human DDT antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DDT and incubated. The DDT found in the sample or standards competes with the biotinylated DDT for limited binding sites on the immobilized anti-human DDT antibody. Excess unbound biotinylated DDT and sample or standard DDT is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DDT captured in the well and inversely proportional to the amount of DDT which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

0.312-20ng/mL

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%

0.12 ng/mL

Store as indicated in product manual.

12 kDa

Natural and recombinant Human D-dopachrome decarboxylase

118

DDT

P30046

DDT