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DDT ELISA Kit (Mouse)

CAT: 0247-OKCD02492-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD02492-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level D-Dopachrome Tautomerase (DDT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level D-Dopachrome Tautomerase (DDT) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
D-dopachrome tautomerase
Gene Aliases
C78655; D-dopachrome decarboxylase; D-dopachrome tautomerase.
Gene ID
13202
Accession Number
NP_034157.1
Reactivity
Mouse|Mus musculus
Target
Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-Dopachrome Tautomerase (DDT) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D-Dopachrome Tautomerase (DDT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D-Dopachrome Tautomerase (DDT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of D-Dopachrome Tautomerase (DDT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.129 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-DDT Microplate 96 Wells (12 x 8 Well strips) DDT Lyophilized Standard 2 100X Biotinylated DDT Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL Component Amount Component Amount Anti-DDT Microplate 96 Wells (12 x 8 Well strips) Anti-DDT Microplate 96 Wells (12 x 8 Well strips) DDT Lyophilized Standard 2 DDT Lyophilized Standard 2 100X Biotinylated DDT Detector Antibody 1 x 120 uL 100X Biotinylated DDT Detector Antibody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL
Specificity
This assay has high sensitivity and excellent specificity for detection of D-Dopachrome Tautomerase (DDT) . No significant cross-reactivity or interference between D-Dopachrome Tautomerase (DDT) and analogues was observed.
Protein Length
118
NCBI Gene Symbol
Ddt
Protein Name
D-dopachrome decarboxylase
Gene Name URL
Ddt
Nucleotide Accession Number
NM_010027.1

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