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ACE ELISA Kit (Rat)

CAT: 0247-OKEH00008-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH00008-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Angiotensin I converting enzyme
Gene Aliases
Angiotensin 1 converting enzyme 1; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; angiotensin I converting enzyme 1; angiotensin I-converting enzyme (Dipeptidyl carboxypeptidase 1) ; angiotensin-converting enzyme; angiotensin-converting enzyme-like; CD143; Dcp1; Dipeptidyl carboxypeptidase 1 (Angiotensin I-converting enzyme) ; dipeptidyl carboxypeptidase I; kininase II; StsRR92.
Gene ID
24310
Accession Number
NP_036676
Reactivity
Rat, Rattus norvegicus
Target
Angiotensin-converting enzyme
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ACE ELISA Kit (Rat) (OKEH00008) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACE captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.157 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
151 kDa
Specificity
Natural and recombinant Rat Angiotensin-converting enzyme
Protein Length
1313
NCBI Gene Symbol
Ace
Protein Name
P47820
Gene Name URL
Ace
Nucleotide Accession Number
NM_012544

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