ACE ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (ng/mL) 0.85 2.73 6.87 0.97 3.42 6.33 St. Dev. 0.036 0.112 0.261 0.671 0.246 0.335 %CV 4.23 4.10 3.79 69.1 7.19 5.29 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (ng/mL) 0.85 2.73 6.87 0.97 3.42 6.33 Mean (ng/mL) 0.85 2.73 6.87 0.97 3.42 6.33 St. Dev. 0.036 0.112 0.261 0.671 0.246 0.335 St. Dev. 0.036 0.112 0.261 0.671 0.246 0.335 %CV 4.23 4.10 3.79 69.1 7.19 5.29 %CV 4.23 4.10 3.79 69.1 7.19 5.29

7732-18-5

Angiotensin I converting enzyme

ACE 1, ACE T, Angiotensin converting enzyme, Angiotensin Converting Enzyme 1, Angiotensinconverting enzyme, Angiotensin-converting enzyme, CD143 antigen, DCP 1, DCP, DCP1, Dipeptidyl carboxypeptidase 1, Dipeptidyl carboxypeptidase I, Kininase II, MVCD3, P12821, Peptidyl dipeptidase A

1636

NP_000780.1

Human

Expression system for standard: NS0; Immunogen sequence: L30-L1261

Angiotensin-convwerting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half of C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073.1 Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm.2 The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

ELISA Kit

ELISA

Aviva's human ACE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for ACE has been precoated onto 96-well plates. Standards (NS0, L30-L1261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ACE amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Cell culture supernates, serum, plasma (heparin) and saliva

0.78 ng/ml - 50 ng/ml

<5 pg/ml

No detectable cross-reactivity with any other cytokine.

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Natural and recombinant human ACE

ACE

Angiotensin-converting enzyme

ACE