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Insulin ELISA Kit (Rabbit)

CAT: 0247-OKCA00228-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00228-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (μIU/mL) 32.982 31.66 30.743 33.576 30.947 32.779 SD 0.021 0.022 0.023 0.03 0.029 0.029 CV% 6.441 7.115 7.638 9.497 9.556 9.097 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (μIU/mL) 32.982 31.66 30.743 33.576 30.947 32.779 Mean (μIU/mL) 32.982 31.66 30.743 33.576 30.947 32.779 SD 0.021 0.022 0.023 0.03 0.029 0.029 SD 0.021 0.022 0.023 0.03 0.029 0.029 CV% 6.441 7.115 7.638 9.497 9.556 9.097 CV% 6.441 7.115 7.638 9.497 9.556 9.097
CAS Number
7732-18-5
Gene Name
Insulin
Gene ID
100009181
Accession Number
NP_001075804.1
Reactivity
Rabbit
Target
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Insulin ELISA Kit (Rabbit) (OKCA00228) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Rabbit monoclonal antibody specific for Rabbit Insulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Rabbit Insulin is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Rabbit Insulin captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates
Detection Range
8 uIU/mL - 140 uIU/mL
Sensitivity
5 uIU/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
12 kDa
Protein Length
110
NCBI Gene Symbol
Insulin
Protein Name
Insulin
Gene Name URL
Insulin
Nucleotide Accession Number
NM_001082335.1

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