Insulin ELISA Kit

• Reproducibility:

  Sample Insulin Conc. (pg/mL) %CV 1 1,013.5 1.5 2 665.3 3.2 3 371.8 2.4 Sample Insulin Conc. (pg/mL) %CV Sample Insulin Conc. (pg/mL) %CV 1 1,013.5 1.5 1 1,013.5 1.5 2 665.3 3.2 2 665.3 3.2 3 371.8 2.4 3 371.8 2.4 Sample Insulin Conc. (pg/mL) %CV 1 655.7 8.9 2 348.7 10.6 3 382.6 11.6 4 340.8 8.8 5 637.6 6.6 6 1,039.5 5.3 Sample Insulin Conc. (pg/mL) %CV Sample Insulin Conc. (pg/mL) %CV 1 655.7 8.9 1 655.7 8.9 2 348.7 10.6 2 348.7 10.6 3 382.6 11.6 3 382.6 11.6 4 340.8 8.8 4 340.8 8.8 5 637.6 6.6 5 637.6 6.6 6 1,039.5 5.3 6 1,039.5 5.3

• Gene Name:

  Insulin

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked immunosorbent assay

• Assay Principle:

  The DetectX® Insulin EIA kit is designed to quantitatively measure insulin present in a variety samples and tissue culture media. Please read the complete kit insert before performing this assay. An Insulin standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture insulin present in the sample. After a 60 minute incubation, the plate is washed. A peroxidase conjugated insulin monoclonal antibody is added and the plate is again incubated for 30 minutes and washed. Substrate is then added to the plate, which reacts with the bound insulin conjugated antibody. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the Insulin in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma and Tissue Culture Media

• Sensitivity:

  48.8 pg/mL

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Clear Coated 96 Well Plate 1 Plate Human Insulin Standard 40 uL DetectX® Insulin Conjugate 5 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL Stop Solution 5 mL Plate Sealer 2 each Component Quantity Component Quantity Clear Coated 96 Well Plate 1 Plate Clear Coated 96 Well Plate 1 Plate Human Insulin Standard 40 uL Human Insulin Standard 40 uL DetectX® Insulin Conjugate 5 mL DetectX® Insulin Conjugate 5 mL Assay Buffer Concentrate 28 mL Assay Buffer Concentrate 28 mL Wash Buffer Concentrate 30 mL Wash Buffer Concentrate 30 mL TMB Substrate 11 mL TMB Substrate 11 mL Stop Solution 5 mL Stop Solution 5 mL Plate Sealer 2 each Plate Sealer 2 each

• CAS Number:

  7732-18-5