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Insulin ELISA Kit (Monkey)

CAT: 0247-OKCA00217-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA00217-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (μIU/mL) 33.659 32.929 30.349 30.07 32.013 29.228 SD 0.023 0.025 0.025 0.031 0.033 0.029 CV% 6.354 7.018 7.651 9.471 9.346 9.187 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (μIU/mL) 33.659 32.929 30.349 30.07 32.013 29.228 Mean (μIU/mL) 33.659 32.929 30.349 30.07 32.013 29.228 SD 0.023 0.025 0.025 0.031 0.033 0.029 SD 0.023 0.025 0.025 0.031 0.033 0.029 CV% 6.354 7.018 7.651 9.471 9.346 9.187 CV% 6.354 7.018 7.651 9.471 9.346 9.187
CAS Number
7732-18-5
Reactivity
Monkey
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Insulin ELISA Kit (Monkey) (OKCA00217) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Monkey monoclonal antibody specific for Monkey Insulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Monkey Insulin is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Monkey Insulin captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma
Detection Range
8 uIU/mL - 140 uIU/mL
Sensitivity
5 uIU/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
Insulin
Gene Name URL
Insulin

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