ELANE ELISA Kit (Human)

CAT: 0247-OKBB01431-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB01431-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 466 1663 9923 528 1713 9943 Standard deviation 22.37 86.48 535.84 26.93 94.22 626.41 CV (%) 4.8 5.2 5.4 5.1 5.5 6.3 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 466 1663 9923 528 1713 9943 Mean (pg/ml) 466 1663 9923 528 1713 9943 Standard deviation 22.37 86.48 535.84 26.93 94.22 626.41 Standard deviation 22.37 86.48 535.84 26.93 94.22 626.41 CV (%) 4.8 5.2 5.4 5.1 5.5 6.3 CV (%) 4.8 5.2 5.4 5.1 5.5 6.3
CAS Number
7732-18-5
Gene Name
Elastase, neutrophil expressed
Gene Aliases
Neutrophil elastase; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase; ELANE; ELA2
Gene ID
1991
Accession Number
NP_001963.1
Reactivity
Human
Immunogen
Expression system for standard: NS0; Immunogen sequence: I30-H267
Target
In humans, neutrophil elastase is encoded by the ELANE gene, which resides on chromosome 19. It is mapped to 19p13.3. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN) . This gene is present in a gene cluster on chromosome 19.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human ELANE ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ELANE has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: I30-H267) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ELANE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ELANE amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
0.78 ng/ml - 50 ng/ml
Sensitivity
<50 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human ELANE
NCBI Gene Symbol
ELANE
Protein Name
Neutrophil elastase
Gene Name URL
ELANE
Nucleotide Accession Number
NM_001972.3

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