ELANE ELISA Kit (Mouse)

• Reproducibility:

  Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 97 301 903 95 322 978 Standard deviation 7.66 15.95 69.53 7.69 19.32 76.28 CV (%) 7.9 5.3 7.7 8.1 6 7.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 97 301 903 95 322 978 Mean (pg/ml) 97 301 903 95 322 978 Standard deviation 7.66 15.95 69.53 7.69 19.32 76.28 Standard deviation 7.66 15.95 69.53 7.69 19.32 76.28 CV (%) 7.9 5.3 7.7 8.1 6 7.8 CV (%) 7.9 5.3 7.7 8.1 6 7.8

• Gene Name:

  Elastase, neutrophil expressed

• Gene Aliases:

  Elastase, Elastase2, Elastase 2, Elastase-2, Ela2, Ela 2, Ela-2, Elane, Leukocyte elastase, Neutrophil elastase

• Gene ID:

  50701

• Accession Number:

  NP_056594.2

• Reactivity:

  Mouse

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: S27-R260

• Target:

  Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN) . This gene is present in a gene cluster on chromosome 19.

• Type:

  ELISA Kit

• Applications:

  ELISA

• Assay Principle:

  Aviva's mouse Elane ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Elane has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: S27-R260) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Elane is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Elane amount of sample captured in plate.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <10 pg/ml

• Reconstitution:

  Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

• Specificity:

  Natural and recombinant mouse Elane

• NCBI Gene Symbol:

  ELANE

• Protein Name:

  Neutrophil elastase

• Gene Name URL:

  ELANE

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_015779.2