ELANE ELISA Kit (Mouse)

CAT: 0247-OKEH00276-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH00276-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Elastase, neutrophil expressed
Gene Aliases
El; Ela2; elastase 2, neutrophil; Elastase-2; F430011M15Rik; leukocyte elastase; N; NE; neutrophil elastase.
Gene ID
50701
Accession Number
NP_056594.2
Reactivity
Mouse, Mus musculus
Target
Neutrophil elastase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ELANE ELISA Kit (Mouse) (OKEH00276) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ELANE has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ELANE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ELANE captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
0.089 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
29 kDa
Specificity
Natural and recombinant Mouse Neutrophil elastase
Protein Length
265
NCBI Gene Symbol
Elane
Protein Name
Q3UP87
Gene Name URL
Elane
Nucleotide Accession Number
NM_015779.2

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