CHRD ELISA Kit (Mouse)

CAT: 0247-OKBB00962-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
CAT#:0247-OKBB00962-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 550 1939 139 561 1941 Standard deviation 10.37 34.65 81.43 12.78 44.31 100.93 CV (%) 7.8 6.3 4.2 9.2 7.9 5.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 133 550 1939 139 561 1941 Mean (pg/ml) 133 550 1939 139 561 1941 Standard deviation 10.37 34.65 81.43 12.78 44.31 100.93 Standard deviation 10.37 34.65 81.43 12.78 44.31 100.93 CV (%) 7.8 6.3 4.2 9.2 7.9 5.2 CV (%) 7.8 6.3 4.2 9.2 7.9 5.2
CAS Number
7732-18-5
Gene Name
Chordin
Gene Aliases
Chordin, Chrd, CHRD_HUMAN, MGC133038, Shortgastrulation protein, SOG
Gene ID
12667
Accession Number
NP_001264970.1
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: T27-S948
Target
Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse Chordin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Chordin has been precoated onto 96-well plates. Standards (NS0, T27-S948) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chordin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Chordin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse Chordin
NCBI Gene Symbol
CHRD
Protein Name
Chordin
Gene Name URL
CHRD
Nucleotide Accession Number
NM_001278041.1

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