CHRD ELISA Kit (Human)

• Reproducibility :

  Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Chordin (CHRD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Chordin (CHRD) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

• CAS Number :

  7732-18-5

• Gene Name :

  Chordin

• Gene Aliases :

  Chordin.

• Gene ID :

  8646

• Accession Number :

  NP_001291401

• Reactivity :

  Homo sapiens|Human

• Target :

  Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes (By similarity) .

• Type :

  ELISA Kit

• Applications :

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method :

  Colorimetric

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chordin (CHRD) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chordin (CHRD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chordin (CHRD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chordin (CHRD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Assay Protocol :

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type :

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range :

  0.312-20ng/mL

• Recovery :

  Matrix Recovery Range (%) Average (%) _x005F serum (n=5) 85-97 89 _x005F EDTA plasma (n=5) 90-98 94 _x005F heparin plasma (n=5) 88-105 99 _x005F Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 85-97 89 serum (n=5) 85-97 89 EDTA plasma (n=5) 90-98 94 EDTA plasma (n=5) 90-98 94 heparin plasma (n=5) 88-105 99 heparin plasma (n=5) 88-105 99

• Sensitivity :

  < 0.115 ng/mL

• Reconstitution :

  2°C to 8°C|-20°C

• Components :

  Component Amount _x005F Anti-CHRD Microplate 96 Wells (12 x 8 Well Strips) _x005F CHRD Lyophilized Standard 2 x 20 ng _x005F 100X Biotinylated CHRD Detector Antibody 120 uL _x005F Avidin/HRP Conjugate 120 uL _x005F Standard Diluent 1 x 20 mL _x005F Detector Antibody Diluent 1 x 12 mL _x005F Conjugate Diluent 1 x 12 mL _x005F 30X Wash Buffer 1 x 20 mL _x005F TMB Substrate 1 x 9 mL _x005F Stop Solution 1 x 6 mL _x005F Component Amount Component Amount Anti-CHRD Microplate 96 Wells (12 x 8 Well Strips) Anti-CHRD Microplate 96 Wells (12 x 8 Well Strips) CHRD Lyophilized Standard 2 x 20 ng CHRD Lyophilized Standard 2 x 20 ng 100X Biotinylated CHRD Detector Antibody 120 uL 100X Biotinylated CHRD Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL

• Notes :

  Formerly GWB-KBBQR1

• Specificity :

  This assay has high sensitivity and excellent specificity for detection of Chordin (CHRD) ._x005F No significant cross-reactivity or interference between Chordin (CHRD) and analogues was observed.

• Protein Length :

  955

• NCBI Gene Symbol :

  CHRD

• Protein Name :

  Chordin

• Gene Name URL :

  CHRD

• Nucleotide Accession Number :

  NM_001304472.1