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Chrd ELISA Kit (Rat)

CAT: 0247-OKBB01453-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB01453-96WSize:96 Well With Removable Strips
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 293 1596 100 395 1791 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 CV (%) 5.1 5.3 5.6 6.6 6.8 7.1 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 95 293 1596 100 395 1791 Mean (pg/ml) 95 293 1596 100 395 1791 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 Standard deviation 4.85 15.53 89.38 6.60 26.86 127.16 CV (%) 5.1 5.3 5.6 6.6 6.8 7.1 CV (%) 5.1 5.3 5.6 6.6 6.8 7.1
CAS Number
7732-18-5
Gene Name
Chordin
Gene Aliases
Chordin; Chrd
Gene ID
117275
Reactivity
Rat
Immunogen
Expression system for standard: NS0; Immunogen sequence: T27-S948
Target
Chordin is a bone morphogenetic protein antagonist composed of four small cysteine-rich domains, whose function is not known. In humans, the chordin peptide is encoded by the CHRD gene. It is mapped to 11q23. It dorsalizes the developing embryo by binding ventralizing TGFβ proteins such as bone morphogenetic proteins. It may also play a role in organogenesis. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's rat Chordin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Chordin has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: T27-S948) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chordin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat Chordin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<50 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant rat Chordin
NCBI Gene Symbol
CHRD
Protein Name
Chordin
Gene Name URL
CHRD

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