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PLTP ELISA Kit (Mouse)

CAT: 0247-OKBB00625-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0247-OKBB00625-96WSize:96 Well With Removable Strips
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Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 9.4 18.5 27 11.2 17.9 31 SD 0.498 0.907 1.134 0.75 0.931 1.49 CV% 5.3 4.9 4.2 6.7 5.2 4.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 9.4 18.5 27 11.2 17.9 31 Mean (pg/mL) 9.4 18.5 27 11.2 17.9 31 SD 0.498 0.907 1.134 0.75 0.931 1.49 SD 0.498 0.907 1.134 0.75 0.931 1.49 CV% 5.3 4.9 4.2 6.7 5.2 4.8 CV% 5.3 4.9 4.2 6.7 5.2 4.8
CAS Number
7732-18-5
Gene Name
Phospholipid transfer protein
Gene Aliases
BPI fold containing family E, BPIFE, HDLCQ9, High absorbance lipoprotein cholesterol level quantitative trait locus 9, included, Lipid transfer protein II, OD107, Phospholipid transfer protein, Plasma phospholipid transfer protein, Pltp, PLTP_HUMAN
Gene ID
18830
Accession Number
NP_035255.1
Reactivity
Mouse
Immunogen
Expression system for standard: NS0; Immunogen sequence: E18-A493
Target
Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL) . In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse PLTP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PLTP has been precoated onto 96-well plates. Standards (NS0, E18-A493) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLTP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse PLTP amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum, plasma (heparin, EDTA)
Detection Range
0.78 ng/ml - 50 ng/ml
Sensitivity
<20 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse PLTP
NCBI Gene Symbol
PLTP
Protein Name
Phospholipid transfer protein
Gene Name URL
PLTP
Nucleotide Accession Number
NM_011125.2

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