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PLTP ELISA Kit (Rat)

CAT: 0247-OKCD04388-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD04388-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Phospholipid Transfer Protein (PLTP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Phospholipid Transfer Protein (PLTP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Phospholipid transfer protein
Gene Aliases
BPI fold containing family E; Bpife; phospholipid transfer protein.
Gene ID
296371
Accession Number
NP_001162015.1
Reactivity
Rat|Rattus norvegicus
Target
Phospholipid transfer protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipid Transfer Protein (PLTP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipid Transfer Protein (PLTP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipid Transfer Protein (PLTP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phospholipid Transfer Protein (PLTP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
3.91-250ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 96-105 101 EDTA Plasma (n=5) 95-105 101 Heparin Plasma (n=5) 95-102 98 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 96-105 101 Serum (n=5) 96-105 101 EDTA Plasma (n=5) 95-105 101 EDTA Plasma (n=5) 95-105 101 Heparin Plasma (n=5) 95-102 98 Heparin Plasma (n=5) 95-102 98
Sensitivity
1.4 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Pltp Microplate 96 Wells (12 x 8 Well strips) Pltp Lyophilized Standard 2 100X Pltp HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Pltp Microplate 96 Wells (12 x 8 Well strips) Pltp Microplate 96 Wells (12 x 8 Well strips) Pltp Lyophilized Standard 2 Pltp Lyophilized Standard 2 100X Pltp HRP-Detector Anitbody 1 x 120 uL 100X Pltp HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
NCBI Gene Symbol
Pltp
Protein Name
Phospholipid transfer protein
Gene Name URL
Pltp
Nucleotide Accession Number
NM_001168543.1

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