PLTP ELISA Kit (Human)

• Reproducibility:

  Mean Inter-Assay Variation

• Gene Aliases:

  BPIFE, HDLCQ9

• Gene ID:

  5360

• Accession Number:

  NP_001229849

• Reactivity:

  Human

• Target:

  Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology PLTP ELISA Kit (Human) (OKCA02131) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLTP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLTP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PLTP captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Tissue Homogenates

• Detection Range:

  2.34 ng/mL-150 ng/mL

• Recovery:

  Sample Type Mean (%) Range (%) Serum (n=5) 99 93-103 EDTA plasma (n=4) 97 90-101 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 99 93-103 Serum (n=5) 99 93-103 EDTA plasma (n=4) 97 90-101 EDTA plasma (n=4) 97 90-101

• Sensitivity:

  0.58 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• NCBI Gene Symbol:

  PLTP

• Protein Name:

  Phospholipid transfer protein

• Gene Name URL:

  PLTP

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001242920