IL-29 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 517 1446 2336 643 1550 2468 SD 33.1 76.64 114.5 45.01 105.4 140.7 CV% 6.4 5.3 4.9 7 6.8 5.7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 517 1446 2336 643 1550 2468 Mean (pg/mL) 517 1446 2336 643 1550 2468 SD 33.1 76.64 114.5 45.01 105.4 140.7 SD 33.1 76.64 114.5 45.01 105.4 140.7 CV% 6.4 5.3 4.9 7 6.8 5.7 CV% 6.4 5.3 4.9 7 6.8 5.7

7732-18-5

Interferon, lambda 1

Cytokine ZCYTO21, IFN lambda 1, IFN-lambda-1, IFNL1, IL 29, IL-29, IL29, IL29_HUMAN, Interferon lambda 1, Interferon lambda-1, Interferon, lambda 1, Interleukin 29, Interleukin-29

282618

NP_742152.1

Human

Expression system for standard: NS0; Immunogen sequence: G20-T200

Interferon lambda-1, also called Interleukin-29 (IL-29) is a protein that in humans is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.

ELISA Kit

ELISA

Aviva's human IL-29 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-29 has been precoated onto 96-well plates. Standards (NS0, G20-T200) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-29 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-29 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

62.5 pg/ml - 4,000 pg/ml

<10 pg/ml

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Natural and recombinant human IL-29

IFNL1

Interferon lambda-1

IFNL1