Products for Research Use Only
RegisterLogin

IL-8 ELISA Kit (Human)

CAT: 0247-OKBB00192-96WSize: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKBB00192-96WSize:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 115 357 616 176 392 649 St. Dev. 6.67 17.5 41.3 13.38 21.17 45.43 %CV 5.8 4.90 6.70 7.60 5.40 7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 115 357 616 176 392 649 Mean (pg/mL) 115 357 616 176 392 649 St. Dev. 6.67 17.5 41.3 13.38 21.17 45.43 St. Dev. 6.67 17.5 41.3 13.38 21.17 45.43 %CV 5.8 4.90 6.70 7.60 5.40 7 %CV 5.8 4.90 6.70 7.60 5.40 7
CAS Number
7732-18-5
Gene Name
Chemokine (C-X-C motif) ligand 8
Gene Aliases
(Ala-IL-8) 77, (Ser-IL-8) 72, 3 10C, 3-10C, AMCF I, AMCF-I, AMCFI, b ENAP, B-ENAP, Beta-thromboglobulin-like protein, C X C motif chemokine 8, C-X-C motif chemokine 8, Chemokine (C X C motif) ligand 8, CXCL8, Emoctakin, GCP 1, GCP-1, GCP/IL-8 protein I, GCP/IL-8 protein II, GCP/IL-8 protein III, GCP/IL-8 protein IV, GCP/IL-8 protein V, GCP/IL-8 protein VI, GCP1, Granulocyte chemotactic protein 1, IL 8, IL-8, IL-8 (1-77), IL-8 (9-77), IL8, IL8/NAP1 form I, IL8/NAP1 form II, IL8/NAP1 form III, IL8/NAP1 form IV, IL8/NAP1 form V, IL8/NAP1 form VI, IL8_HUMAN, Interleukin 8, K60, LECT, LUCT, Lymphocyte derived neutrophil activating factor, Lymphocyte-derived neutrophil-activating factor, LYNAP, MDNCF, MDNCF-b, MDNCF-c, MONAP, Monocyte derived neutrophil activating peptide, Monocyte derived neutrophil chemotactic factor, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAF, NAP 1, NAP-1, NAP1, Neutrophil activating peptide 1, Neutrophil activating protein 1, Neutrophil-activating factor, Neutrophil-activating protein 1, Protein 3 10C, Protein 3-10C, SCYB8, Small inducible cytokine subfamily B member 8, T cell chemotactic factor, T-cell chemotactic factor, TSG 1, TSG-1, TSG1
Gene ID
3576
Accession Number
NP_000575.1
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S28-S99
Target
Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils.1 IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications.2 The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside.1 Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human IL-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-8 has been precoated onto 96-well plates. Standards (E.coli, S28-S99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-8 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Homology
No detectable cross-reactivity with any other cytokine.
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human IL-8
NCBI Gene Symbol
CXCL8
Protein Name
Interleukin-8
Gene Name URL
CXCL8
Nucleotide Accession Number
NM_000584.3

Related Products

CatalogName
OKWB00306IL-8 ELISA Kit (Monkey) (OKWB00306)
OKRC01367-96WOKRC01367-96W - IL-8 ELISA Kit (Pig)
OKRC01078-96WOKRC01078-96W - IL-8 ELISA Kit (Rabbit)
OKRC00098-96WOKRC00098-96W - IL-8 ELISA Kit (Cat)
OKRC00068-96WOKRC00068-96W - IL-8 ELISA Kit (Dog)
OKRC01058-96WOKRC01058-96W - IL-8 ELISA Kit (Rhesus Macaque)
OPPA00440-10UGIL 8 Protein
OPPA00424-5UGIL 8 Protein
OADB00383-500UGIL-8 Antibody
OAPA00012-500UGIL-8 Antibody

Alternative Products

CatalogName
DIY1672H-01Human IL-8 ELISA
YP-W15456-01Human IL-8; CXCL8 (IL-8; CXCL8) ELISA Kit
K4169-100IL-8 (Human) ELISA Kit
MBS335478-01Human IL-8 ELISA Kit
KET6018-01ELISA kit for Human IL-8
3560-1HP-1ELISA Pro: Human IL-8
E2253IL-8) Human ELISA Kit
ADI-900-156AIL-8 (human), ELISA kit

Popular Products