Galactose Colorimetric Detection Kit

• Reproducibility:

  Sample Galactose Conc. (mg/dL) %CV 1 9.58 4.8 2 5.75 3.6 3 3.18 6.2 Sample Galactose Conc. (mg/dL) %CV Sample Galactose Conc. (mg/dL) %CV 1 9.58 4.8 1 9.58 4.8 2 5.75 3.6 2 5.75 3.6 3 3.18 6.2 3 3.18 6.2 Sample Galactose Conc. (mg/dL) %CV 1 8.50 8.4 2 5.41 5.1 3 2.95 4.8 Sample Galactose Conc. (mg/dL) %CV Sample Galactose Conc. (mg/dL) %CV 1 8.50 8.4 1 8.50 8.4 2 5.41 5.1 2 5.41 5.1 3 2.95 4.8 3 2.95 4.8

• Gene Name:

  Galactose Colorimetric

• Type:

  Application Kit

• Detection Method:

  Colorimetric

• Assay Principle:

  The DetectX® Galactose Colorimetric Detection Kit is designed to quantitatively measure galactose in a variety of samples. Please read the complete kit insert before performing this assay. A D- (+) -galactose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Substrate and horseradish peroxidase and the reaction initiated by addition of galactose oxidase. The plate is incubated at room temperature for 30 minutes. The galactose oxidase reacts with galactose to produce hydrogen peroxide which, in the presence of HRP, reacts with the colorless Substrate to produce a pink-colored product to be read at 560 nm. Increasing levels of galactose cause a linear increase in color.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Buffers and TCM

• Sensitivity:

  0.493 mg/dL

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Clear Half Area 96 Well Plates 2 Plates Galactose Standard 90 uL Assay Buffer 50 mL Substrate 5 mL Horseradish Peroxidase Concentrate 60 uL Galactose Oxidase 2 Vials Component Quantity Component Quantity Clear Half Area 96 Well Plates 2 Plates Clear Half Area 96 Well Plates 2 Plates Galactose Standard 90 uL Galactose Standard 90 uL Assay Buffer 50 mL Assay Buffer 50 mL Substrate 5 mL Substrate 5 mL Horseradish Peroxidase Concentrate 60 uL Horseradish Peroxidase Concentrate 60 uL Galactose Oxidase 2 Vials Galactose Oxidase 2 Vials