Thiol Fluorescent Detection Kit

• Reproducibility:

  Sample Thiol Concentration (nM) %CV 1 272.4 3.0 2 801.4 2.4 3 1,128 2.2 4 2,226 2.4 Sample Thiol Concentration (nM) %CV Sample Thiol Concentration (nM) %CV 1 272.4 3.0 1 272.4 3.0 2 801.4 2.4 2 801.4 2.4 3 1,128 2.2 3 1,128 2.2 4 2,226 2.4 4 2,226 2.4 Sample Thiol Concentration (nM) %CV 1 319.2 8.6 2 940.3 10.8 3 1,246 8.7 4 2,423 6.2 Sample Thiol Concentration (nM) %CV Sample Thiol Concentration (nM) %CV 1 319.2 8.6 1 319.2 8.6 2 940.3 10.8 2 940.3 10.8 3 1,246 8.7 3 1,246 8.7 4 2,423 6.2 4 2,423 6.2

• Gene Name:

  Thiol

• Type:

  Application Kit

• Detection Method:

  Fluorometric

• Assay Principle:

  The DetectX® Thiol Kit is designed to quantitatively measure thiol groups generated or present in biological samples. Please read the complete kit insert before performing the assay. A standard is provided to generate a standard curve for the assay and all samples should be read off the curve generated. Samples and standards are pipetted into a black microtiter plate. After mixing the sample or standard with ThioStar and incubating at room temperature for a 30 minute incubation, the fluorescent product is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. The concentration of the thiol in the sample is calculated, after making a suitable correction for any dilution of the sample, using software available with most fluorescence plate readers. We have provided a 96 well plate for measurement but this assay is adaptable for measurement in higher density plate formats. The end user should ensure that their black plate is suitable for use with these reagents prior to running samples. Since biologically generated free thiols, such as glutathione, and protein thiol groups, exist in different environments we suggest that the end user calibrate the amount of thiol present or generated using a suitable standard.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Proteins and Peptides in Biological Buffers

• Sensitivity:

  4.62 nM

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Black Half Area 96 Well Plate 96 Well N-Acetylcysteine Standard 220 uL ThioStar® Detection Reagent 2 vials Dry DMSO 4 mL Assay Buffer Concentrate 60 mL Plate Sealer 1 each Component Quantity Component Quantity Black Half Area 96 Well Plate 96 Well Black Half Area 96 Well Plate 96 Well N-Acetylcysteine Standard 220 uL N-Acetylcysteine Standard 220 uL ThioStar® Detection Reagent 2 vials ThioStar® Detection Reagent 2 vials Dry DMSO 4 mL Dry DMSO 4 mL Assay Buffer Concentrate 60 mL Assay Buffer Concentrate 60 mL Plate Sealer 1 each Plate Sealer 1 each