BChE Fluorescent Activity Kit

CAT: 0247-OKAU00015-2PLATESize: 2 PlatesDry Ice: NoHazardous: No
CAT#:0247-OKAU00015-2PLATESize:2 Plates
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Reproducibility
Sample BChE Activity (mU/mL) %CV 1 5.70 4.7 2 2.97 7.3 3 1.17 7.5 Sample BChE Activity (mU/mL) %CV Sample BChE Activity (mU/mL) %CV 1 5.70 4.7 1 5.70 4.7 2 2.97 7.3 2 2.97 7.3 3 1.17 7.5 3 1.17 7.5 Sample BChE Activity (mU/mL) %CV 1 7.70 9.1 2 5.84 7.5 3 1.71 8.5 Sample BChE Activity (mU/mL) %CV Sample BChE Activity (mU/mL) %CV 1 7.70 9.1 1 7.70 9.1 2 5.84 7.5 2 5.84 7.5 3 1.71 8.5 3 1.71 8.5
Type
Application Kit
Applications
AA
Detection Method
Fluorometric
Assay Principle
The DetectX® Butyrylcholinesterase Activity Kit is designed to quantitatively measure butyrylcholinesterase (BChE) activity in a variety of samples. Please read the complete kit insert before performing this assay. A human BChE standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. The kit utilizes a proprietary non-fluorescent molecule, ThioStar®, that covalently binds to the thiol product of the reaction between the BChE Substrate and BChE in the standards or samples, yielding a fluorescent product read at 510 nm in a fluorescent plate reader with excitation at 390 nm. The kit is suitable for measuring BChE activity in appropriately diluted serum or plasma from a number of species. It will also measure BChE in extracted tissue samples, CSF and cell lysates. Because the readout of BChE activity is purely chemical, there are few interferants that will affect the readings obtained.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum and Plasma
Sensitivity
0.018 mU/mL
Reconstitution
2°C to 8°C
Components
Component Quantity Black 96 Well Plates 2 Plates Butyrylcholinesterase Standard 225 uL ThioStar® Detection Reagent 2 vials BChE Substrate 2 vials Dry DMSO 14 mL Assay Buffer Concentrate 28 mL Component Quantity Component Quantity Black 96 Well Plates 2 Plates Black 96 Well Plates 2 Plates Butyrylcholinesterase Standard 225 uL Butyrylcholinesterase Standard 225 uL ThioStar® Detection Reagent 2 vials ThioStar® Detection Reagent 2 vials BChE Substrate 2 vials BChE Substrate 2 vials Dry DMSO 14 mL Dry DMSO 14 mL Assay Buffer Concentrate 28 mL Assay Buffer Concentrate 28 mL
NCBI Gene Symbol
BCHE
Gene Name URL
BCHE

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