AChE Fluorescent Activity Kit

• Reproducibility:

  Sample AChE Activity (mU/mL) %CV 1 63.9 8.9 2 41.6 9.1 3 11.5 6.7 Sample AChE Activity (mU/mL) %CV Sample AChE Activity (mU/mL) %CV 1 63.9 8.9 1 63.9 8.9 2 41.6 9.1 2 41.6 9.1 3 11.5 6.7 3 11.5 6.7 Sample AChE Activity (mU/mL) %CV 1 59.1 7.9 2 39.9 8.5 3 11.3 11.1 Sample AChE Activity (mU/mL) %CV Sample AChE Activity (mU/mL) %CV 1 59.1 7.9 1 59.1 7.9 2 39.9 8.5 2 39.9 8.5 3 11.3 11.1 3 11.3 11.1

• Type:

  Application Kit

• Applications:

  AA

• Detection Method:

  Fluorometric

• Assay Principle:

  The DetectX® Acetylcholinesterase Activity Kit is designed to quantitatively measure acetylcholinesterase (AChE) activity in a variety of samples. Please read the complete kit insert before performing this assay. A human AChE standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. The kit utilizes a proprietary non-fluorescent molecule, ThioStar®, that covalently binds to the thiol product of the reaction between the AChE Substrate and AChE in the standards or samples, yielding a fluorescent product read at 510 nm in a fluorescent plate reader with excitation at 390 nm. The kit is suitable for measuring AchE activity in appropriately diluted serum, plasma and RBC ghosts from a number of species. It will also measure AChE in extracted tissue samples and cell lysates. Because the readout of AChE activity is purely chemical, there are few interferants that will affect the readings obtained.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, and Erythrocyte Membranes

• Sensitivity:

  0.218 mU/mL

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Black 96 Well Plates 2 Plates Acetylcholinesterase Standard 225 uL ThioStar® Detection Reagent 2 vials Dry DMSO 14 mL Assay Buffer Concentrate 28 mL AChE Substrate 2 vials Component Quantity Component Quantity Black 96 Well Plates 2 Plates Black 96 Well Plates 2 Plates Acetylcholinesterase Standard 225 uL Acetylcholinesterase Standard 225 uL ThioStar® Detection Reagent 2 vials ThioStar® Detection Reagent 2 vials Dry DMSO 14 mL Dry DMSO 14 mL Assay Buffer Concentrate 28 mL Assay Buffer Concentrate 28 mL AChE Substrate 2 vials AChE Substrate 2 vials

• NCBI Gene Symbol:

  ACHE

• Gene Name URL:

  ACHE