BCHE ELISA Kit (Rat)

CAT: 0247-OKCD00683-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKCD00683-96WSize:96 Well
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Butyrylcholinesterase (BCHE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Butyrylcholinesterase (BCHE) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Butyrylcholinesterase
Gene Aliases
Cholinesterase.
Gene ID
65036
Accession Number
NP_075231
Reactivity
Rat|Rattus norvegicus
Target
Carboxylic ester hydrolase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Butyrylcholinesterase (BCHE) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Butyrylcholinesterase (BCHE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Butyrylcholinesterase (BCHE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Butyrylcholinesterase (BCHE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
1.56-100ng/mL
Recovery
Matrix Recovery Range (%) Average (%) serum (n=5) 89-96 93 EDTA plasma (n=5) 86-103 94 heparin plasma (n=5) 81-96 91 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) serum (n=5) 89-96 93 serum (n=5) 89-96 93 EDTA plasma (n=5) 86-103 94 EDTA plasma (n=5) 86-103 94 heparin plasma (n=5) 81-96 91 heparin plasma (n=5) 81-96 91
Sensitivity
< 0.63 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-Bche Microplate 96 Wells (12 x 8 Well Strips) Bche Lyophilized Standard 2 x 100 ng 100X Biotinylated Bche Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-Bche Microplate 96 Wells (12 x 8 Well Strips) Anti-Bche Microplate 96 Wells (12 x 8 Well Strips) Bche Lyophilized Standard 2 x 100 ng Bche Lyophilized Standard 2 x 100 ng 100X Biotinylated Bche Detector Antibody 120 uL 100X Biotinylated Bche Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBQG5
Specificity
This assay has high sensitivity and excellent specificity for detection of Butyrylcholinesterase (BCHE) . No significant cross-reactivity or interference between Butyrylcholinesterase (BCHE) and analogues was observed.
Protein Length
597
NCBI Gene Symbol
Bche
Protein Name
Carboxylic ester hydrolase
Gene Name URL
Bche
Nucleotide Accession Number
NM_022942.1

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