Hemoglobin Colorimetric Detection Kit

• Reproducibility:

  Sample Hemoglobin Conc. (g/dL) %CV 1 7.63 1.7 2 4.73 3.1 3 1.34 2.2 Sample Hemoglobin Conc. (g/dL) %CV Sample Hemoglobin Conc. (g/dL) %CV 1 7.63 1.7 1 7.63 1.7 2 4.73 3.1 2 4.73 3.1 3 1.34 2.2 3 1.34 2.2 Sample Hemoglobin Conc. (g/dL) %CV 1 7.48 2.9 2 4.69 3.6 3 1.34 7.9 Sample Hemoglobin Conc. (g/dL) %CV Sample Hemoglobin Conc. (g/dL) %CV 1 7.48 2.9 1 7.48 2.9 2 4.69 3.6 2 4.69 3.6 3 1.34 7.9 3 1.34 7.9

• Gene Name:

  Hemoglobin Colorimetric

• Type:

  Application Kit

• Detection Method:

  Colorimetric

• Assay Principle:

  The DetectX® Hemoglobin detection kit is designed to quantitatively measure all forms of hemoglobin present in blood and RBCs. Please read the complete kit insert before performing this assay. A human hemoglobin standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate and the ready-to-use Hemoglobin Detection Reagent is added to each well. 10 uL of samples and standards are used. The plate is incubated for 30 minutes at room temperature. The plate is read at 560-580 nm to detect the intensity of the color generated. Results are calculated as g/dL for whole blood and RBCs. The concentration of the hemoglobin in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers. The DetectX® Hemoglobin Detection kit uses a single reaction solution that is light stable at 4C and does not contain dangerous chemicals. All forms of hemoglobin are rapidly converted to a single stable form that is measured photometrically. Many samples can be measured without dilution in this safe, simple assay.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Whole Blood and RBCs

• Sensitivity:

  0.021 g/dL

• Reconstitution:

  2°C to 8°C

• Components:

  Component Quantity Clear 96 Well Plates 1 plate Hemoglobin Standard 300 uL Hemoglobin Sample Diluent 50 mL Hemoglobin Detection Reagent 20 mL Component Quantity Component Quantity Clear 96 Well Plates 1 plate Clear 96 Well Plates 1 plate Hemoglobin Standard 300 uL Hemoglobin Standard 300 uL Hemoglobin Sample Diluent 50 mL Hemoglobin Sample Diluent 50 mL Hemoglobin Detection Reagent 20 mL Hemoglobin Detection Reagent 20 mL