Urea Nitrogen Detection Kit

CAT: 0247-OKAU00022-10PLATESize: 10 PlatesDry Ice: NoHazardous: No
CAT#:0247-OKAU00022-10PLATESize:10 Plates
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Sample Urea Nitrogen Conc. (mg/dL) %CV 1 1.24 2.0 2 2.29 1.9 3 4.86 2.8 Sample Urea Nitrogen Conc. (mg/dL) %CV Sample Urea Nitrogen Conc. (mg/dL) %CV 1 1.24 2.0 1 1.24 2.0 2 2.29 1.9 2 2.29 1.9 3 4.86 2.8 3 4.86 2.8 Sample Urea Nitrogen Conc. (mg/dL) %CV 1 1.29 3.1 2 2.35 4.3 3 5.18 3.3 Sample Urea Nitrogen Conc. (mg/dL) %CV Sample Urea Nitrogen Conc. (mg/dL) %CV 1 1.29 3.1 1 1.29 3.1 2 2.35 4.3 2 2.35 4.3 3 5.18 3.3 3 5.18 3.3
Gene Name
Urea Nitrogen
Type
Application Kit
Assay Principle
The DetectX® Urea Nitrogen (also called BUN) Detection Kit is designed to quantitatively measure urea nitrogen in a variety of samples. Please read the complete kit insert before performing this assay. A urea nitrogen standard calibrated to NIST reference materials is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with Color Reagents A and B and incubated at room temperature for 30 minutes. The colored product is read at 450 nm. The concentration of urea nitrogen in the sample is calculated, after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of mg/dL urea nitrogen. If samples are to be expressed in terms of mg/dL urea, the data can be converted using the multiplier 2.14.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Saliva and TCM
Sensitivity
0.030 mg/dL
Reconstitution
RT
Components
Component Quantity Clear 96 Well Plates 2 or 2 by 5 Each Urea Nitrogen Standard 250 uL or 1 mL Color Reagent A 15 mL or 2 by 38 mL Color Reagent B 15 mL or 2 by 38 mL Component Quantity Component Quantity Clear 96 Well Plates 2 or 2 by 5 Each Clear 96 Well Plates 2 or 2 by 5 Each Urea Nitrogen Standard 250 uL or 1 mL Urea Nitrogen Standard 250 uL or 1 mL Color Reagent A 15 mL or 2 by 38 mL Color Reagent A 15 mL or 2 by 38 mL Color Reagent B 15 mL or 2 by 38 mL Color Reagent B 15 mL or 2 by 38 mL

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