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POLR2A Antibody

CAT: 0247-OASG06476-100ULSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0247-OASG06476-100ULSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9007-83-4
Gene Name
Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Gene Aliases
RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, NEDHIB, RpIILS, hsRPB1, hRPB220
Gene ID
5430; 20020
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Synthesized peptide derived from human Rpb1 around the non-phosphorylation site of S1619. Location: 1560-1640aa.
Target
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Clonality
Polyclonal
Type
Polyclonal Antibody
Applications
WB, IHC, IF
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
1 mg/ml
Format
Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Reconstitution
Store at -20C. Avoid repeated freeze/thaw cycles.
Molecular Weight
250 kDa
Fragment
IgG
Specificity
Rpb1 Polyclonal Antibody detects endogenous levels of Rpb1 protein.
NCBI Gene Symbol
POLR2A
Host or Source
Rabbit
Gene Name URL
POLR2A

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