POLR2E Antibody

• Gene Name:

  Polymerase (RNA) II subunit E

• Gene Aliases:

  RPB5, XAP4, RPABC1, hRPB25, hsRPB5

• Gene ID:

  5434

• Swiss Prot:

  P19388

• Accession Number:

  NP_002686.2

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein of human POLR2E

• Target:

  This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC, IF

• Purification:

  Affinity purified against immunogen

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  Liquid

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Reconstitution:

  Store at -20C. Avoid repeated freeze/thaw cycles.

• Molecular Weight:

  24 kDa

• Fragment:

  IgG

• NCBI Gene Symbol:

  POLR2E

• Host or Source:

  Rabbit

• Protein Name:

  DNA-directed RNA polymerases I, II, and III subunit RPABC1

• Gene Name URL:

  POLR2E

• CAS Number:

  9007-83-4

• Nucleotide Accession Number:

  NM_002695.3