Cell Transformation Assay Kit (Fluorometric)

• Description:

  Transformed cells can proliferate without attaching to surface Anchorage-independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). BioVision’s Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit uses a quantitative dye that binds to nucleic acid and generates green fluorescence. This one-step method is non-radioactive and simple (just add-and-read, and does not require tedious labor such as counting colonies). The assay is high-throughput adaptable and has wide linear range from 50-60,000 cells. The entire assay can be finished within 7-8 days.

• Reactivity:

  Mammalian cells

• Applications:

  • Measure cell transformation in response to stimuli • Screen and characterize compounds that influence cell transformation

• Detection Method:

  Fluorescence (Ex/Em = 480/530 nm)

• Features & Benefits:

  • Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation

• Shipping Conditions:

  gel pack

• Storage Conditions:

  -20°C

• Shelf Life:

  12 months