Citrulline Fluorometric Assay Kit

CAT:

26-K2002-100

Size:

100 Assays

Price:

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Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

Citrulline Fluorometric Assay Kit

Description: L-Citrulline is a non-proteogenic, semi-essential amino acid. Citrulline is formed either by ornithine carbamoyltransferase or as a by-product of the Nitric oxide synthase (NOS) activity. Citrulline generated in the NOS reaction can be recycled to arginine by the two enzymes argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL) acting in the Urea Cycle. Citrulline concentrations have been shown to be elevated in patients with mutated ASS or ASL genes. Watermelon is one of the richest sources of Citrulline. Additionally, Citrulline has been advertised as sports nutrition supplement due to its involvement in Nitric Oxide (NO) synthesis, which helps with vasodilation. BioVision’s Citrulline Assay Kit provides a rapid, specific, and easy method for the measurement of total Citrulline concentrations in a wide variety of samples. In this enzymatic assay, Citrulline is converted into a series of intermediates, which further reacts with a probe producing a stable fluorescent signal (Ex/Em = 535/587 nm). The kit is simple, easy to perform, sensitive and is high-throughput adaptable. It can detect as low as 2 µM Citrulline in biological samples.
Reactivity: All
Applications: Analysis of Urea cycle and NO cycle, Measurement of Citrulline in beverages and biological samples.
Detection Method: Fluorescence (Ex/Em = 535/587 nm)
Features & Benefits: Easy method for the measurement of total Citrulline concentrations in a wide variety of samples., Specific, Rapid
Shipping Conditions: Gel Pack
Storage Conditions: -20°C
Shelf Life: 12 months

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