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Cell Transformation Assay Kit (Colorimetric)

CAT: 0026-K921-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K921-100Size:100 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The transformed cells can proliferate without attaching to surface. Anchorage independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). BioVision’s Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit is based on the conversion of the tetrazolium salt (WST-8) to formazan by cellular mitochondrial dehydrogenases. The generated signal is directly proportional to the number of living cells. This one-step method is non-radioactive and simple (just add-and-read, does not require harvesting cells, and solubilization steps). The assay is high-throughput adaptable and has wide linear range from 10000-400000 cells. The entire Cell Transformation Assay can be finished within 7-8 days.
Reactivity
Mammalian cells
Applications
• Measure cell transformation in response to stimuli • Screen and characterize compounds that influence cell transformation
Detection Method
Colorimetric (OD 450 nm)
Features & Benefits
• Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
12 months

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